Journal: Transfusion

Article Title: The feasibility and efficacy of subcutaneous plerixafor for mobilization of peripheral blood stem cells in allogeneic HLA–identical sibling donors: results of the HOVON‐107 study

doi: 10.1111/trf.15037

Figure Lengend Snippet: Composition of the grafts obtained from healthy adult sibling donors

Article Snippet: T lymphocytes were purified from peripheral blood using a human whole blood CD3 positive selection kit (STEMCELL Technologies, Inc.) according to the manufacturer's instructions.

Techniques:

Journal: Transfusion

Article Title: The feasibility and efficacy of subcutaneous plerixafor for mobilization of peripheral blood stem cells in allogeneic HLA–identical sibling donors: results of the HOVON‐107 study

doi: 10.1111/trf.15037

Figure Lengend Snippet: Chimerism in peripheral blood (PB), bone marrow (BM), and CD3 selected cells at 3, 6, and 12 months after transplant

Article Snippet: T lymphocytes were purified from peripheral blood using a human whole blood CD3 positive selection kit (STEMCELL Technologies, Inc.) according to the manufacturer's instructions.

Techniques:

Journal: Transfusion

Article Title: The feasibility and efficacy of subcutaneous plerixafor for mobilization of peripheral blood stem cells in allogeneic HLA–identical sibling donors: results of the HOVON‐107 study

doi: 10.1111/trf.15037

Figure Lengend Snippet: Immunologic reconstitution. Numbers of CD3+; CD3+/CD4+; CD3 + CD8+; CD19+, and NK cells in the peripheral blood at 3, 6, 12, and 24 months after transplant. At 12 months: CD3 + cells: median, 0.84 × 10 9 /L (0.33–1.65); normal range, 0.66–2.10. CD3+/CD4+ cells: median, 0.30 × 10 9 /L (0.17–0.75); normal range, 0. 32–1.36. CD3+/CD8+ cells: median, 0.45 × 10 9 /L (0.11–1.24); normal range, 0.15–0.88. CD3–/CD16+/CD56+ NK cells: median, 0.20 × 10 9 /L (0.13–0.52); normal range, 0.09–0.62. CD19+ cells: median, 0.27 × 10 9 /L (0.09–0.95); normal range, 0.10–0.5. Each box is drawn from the 25th percentile to the 75th percentile of the cell counts, and the horizontal bar within the box indicates the median cell count. The line up from the top of the box goes to the upper adjacent value as defined. The dots indicate cell counts larger than the upper adjacent value. [Color figure can be viewed at wileyonlinelibrary.com ]

Article Snippet: T lymphocytes were purified from peripheral blood using a human whole blood CD3 positive selection kit (STEMCELL Technologies, Inc.) according to the manufacturer's instructions.

Techniques: Cell Counting

Journal: STAR Protocols

Article Title: Protocol for generating human craniofacial cartilage organoids from stem-cell-derived neural crest cells

doi: 10.1016/j.xpro.2024.103532

Figure Lengend Snippet:

Article Snippet: Use EasySep Release Human PSC-derived Neural Crest Cell Positive Selection Kit, Stem cell Technologies, Cat# 100–047 to sort the cells based on cell surface CD271 (NGFR) marker using magnetic beads as described in the following attached kit manufacturer’s protocol: https://cdn.stemcell.com/media/files/pis/10000007951-PIS_00.pdf . j.

Techniques: Recombinant, Knock-Out, Membrane, Selection